The mechanistic study of the effect of methamphetamine and diazepam on antigen-specific antibody production in ovalbumin-sensitized mice
Date Issued
2006
Date
2006
Author(s)
Wu, Hsin-Ying
DOI
en-US
Abstract
Amphetamines and benzodiazepines are commonly abused drugs in Taiwan. The objective of this thesis was to investigate the effects of methamphetamine (MA) and diazepam (DZ), either alone or in combination, on the antigen-specific antibody production in ovalbumin (OVA)-sensitized mice and to study the underlying mechanisms responsible for MA-and DZ-mediated effects on antibody production. BALB/c mice were daily administered with MA and DZ for 5 consecutive days to mimic the status of drug addiction, followed by sensitization with aluminum potassium sulfate-absorbed ovalbumin (OVA/alum). The results demonstrated that repeated dosage of MA and DZ (5 mg/kg), either alone or in combination, significantly attenuated the serum levels of OVA-specific IgM, IgG1 and IgG2a. In accordant with the effect on antibody production, the steady state mRNA expression and protein secretion of IL-4 and IFN-γ by splenocytes isolated from drug-treated and OVA/alum-sensitized mice were attenuated as compared to the vehicle-treated control. In addition, MA and DZ attenuated the proliferation of splenocytes stimulated with OVA or the T-cell mitogen concanavalin A, and increased the percentage of apoptotic splenocytes. MA and DZ moderately decreased expression of T-bet and increased expression of SOCS3. In conclusion, the present studies demonstrated that both MA and DZ administration exhibit marked immunosuppressive effects in OVA/alum-sensitized mice. MA- and DZ-mediated suppression of antigen-specific antibody production is correlated with down regulation of cytokine expression. One of the potential mechanisms of MA- and DZ-mediated immunosuppression is via the inhibition of antigen-specific splenocyte proliferation and the enhancement of antigen-specific splenocyte apoptosis.
Subjects
甲基安非他命
二氮平
抗原專一性免疫反應
免疫球蛋白
細胞激素
卵白蛋白
methamphetamine
diazepam
antigen-specific immune responses
immunoglobulin
cytokine
ovalbumin
SDGs
Type
thesis
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