Avian oncogenic virus differential diagnosis in chickens using oligonucleotide microarray
Journal
Journal of Virological Methods
Journal Volume
210
Pages
45-50
Date Issued
2014
Author(s)
Abstract
Avian oncogenic viruses include the avian leukosis virus (ALV), reticuloendotheliosis virus (REV) and Marek's disease virus (MDV). Multiple oncogenic viral infections are frequently seen, with even Marek's disease vaccines reported to be contaminated with ALV and REV. The gross lesions caused by avian oncogenic viruses often overlap, making differentiation diagnosis based on histopathology difficult. The objective of this study is to develop a rapid approach to simultaneously differentiate, subgroup and pathotype the avian oncogenic viruses. The oligonucleotide microarray was employed in this study. Particular DNA sequences were recognized using specific hybridization between the DNA target and probe on the microarray, followed with colorimetric development through enzyme reaction. With 10 designed probes, ALV-A, ALV-E, ALV-J, REV, MDV pathogenic and vaccine strains were clearly discriminated on the microarray with the naked eyes. The detection limit was 27 copy numbers, which was 10-100 times lower than multiplex PCR. Of 102 field samples screened using the oligonucleotide microarray, 32 samples were positive for ALV-E, 17 samples were positive for ALV-J, 6 samples were positive for REV, 4 samples were positive for MDV, 7 samples were positive for both ALV-A and ALV-E, 5 samples were positive for ALV-A, ALV-E and ALV-J, one sample was positive for both ALV-J and MDV, and 3 samples were positive for both REV and MDV. The oligonucleotide microarray, an easy-to-use, high-specificity, high-sensitivity and extendable assay, presents a potent technique for rapid differential diagnosis of avian oncogenic viruses and the detection of multiple avian oncogenic viral infections under field conditions. ? 2014 Elsevier B.V.
Subjects
Avian leukosis virus; Avian oncogenic viruses; Marek's disease virus; Oligonucleotide microarray; Reticuloendotheliosis virus
Other Subjects
animal tissue; Article; Avian leukosis oncovirus; Avian leukosis oncovirus A; Avian leukosis oncovirus E; Avian leukosis oncovirus J; avian oncogenic virus; chicken; colorimetry; comparative study; controlled study; differential diagnosis; DNA hybridization; DNA microarray; DNA sequence; enzyme mechanism; limit of detection; Mardivirus; multiplex polymerase chain reaction; nonhuman; Reticuloendotheliosis virus; sensitivity and specificity; Taiwan; tumor virus; virus detection; animal; avian leukosis; Avian leukosis virus; bird disease; differential diagnosis; DNA microarray; genetics; isolation and purification; Marek disease; time factor; tumor virus; veterinary; virology; virus infection; Animals; Avian Leukosis; Avian Leukosis Virus; Chickens; Diagnosis, Differential; Limit of Detection; Mardivirus; Marek Disease; Multiplex Polymerase Chain Reaction; Oligonucleotide Array Sequence Analysis; Oncogenic Viruses; Poultry Diseases; Reticuloendotheliosis virus; Time Factors; Tumor Virus Infections
Type
journal article