A hypersensitive response was induced by virulent bacteria in transgenic tobacco plants overexpressing a plant ferredoxin-like protein (PFLP)
Resource
Physiological Molecular Plant Pathology, 64: 103-110
Journal
Physiological Molecular Plant Pathology
Journal Issue
64
Pages
103-110
Date Issued
2004
Date
2004
Author(s)
Abstract
The hypersensitive response (HR) displayed by resistance plants against invading pathogens is a prominent feature of an incompatible plant pathogen interaction. It has been shown that tobacco cell cultures transgenic for a plant ferredoxin-like protein (PFLP) that functions as an electron acceptor of Photosystem I increased harpin-mediate HR. In this work we report increased bacterial disease resistance of pflp transgenic tobacco. Compared to the controls, four distinctive characteristics were found in the pflp-transgenics after inoculation with virulent bacterial cells Erwinia carotovora subsp. carotovora and Pseudomonas syringae pv. tabaci: (i) instead of typical disease symptoms, an HR-like necrosis was observed; (ii) the proliferation of the virulent pathogen was highly retarded; (iii) the expression of hsr203j, an HR marker gene, was apparently induced; (iv)H 2O 2 accumulation was induced immediately. Together, those results demonstrate that enhanced production of PFLP in the transgenic plant conditions the induction of a hypersensitive response during compatible pathogen attack. © 2004 Elsevier Ltd. All rights reserved.
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