Regulation of human endothelial progenitor cell maturation by polyurethane nanocomposites
Journal
Biomaterials
Journal Volume
35
Journal Issue
25
Pages
6810-6821
Date Issued
2014
Author(s)
Hung, H.-S.
Yang, Y.-C.
Lin, Y.-C.
Lin, S.-Z.
Kao, W.-C.
Hsieh, H.-H.
Chu, M.-Y.
Fu, R.-H.
Hsu, S.-H.
Abstract
The mobilization and homing of endothelial progenitor cells (EPCs) are critical to the development of an antithrombotic cardiovascular prosthesis. Polyurethane (PU) with superior elasticity may provide a mechanical environment resembling that of the natural vascular tissues. The topographical cues of PU were maximized by making nanocomposites with a small amount of gold nanoparticles (AuNPs). The nanocomposites of PU-AuNPs ("PU-Au") with a favorable response of endothelial cells were previously established. In the current study, the effect of PU and PU-Au nanocomposites on the behavior of human peripheral blood EPCs was investigated invitro and invivo. It was found that PU-Au promoted EPCs to become differentiated endothelial cells invitro, confirmed by the increased expressions of CD31 and VEGF-R2 surface markers. The increased maturation of EPCs was significantly more remarkable on PU-Au, probably through the stromal derived factor 1α (SDF-1α)/CXCR4 signaling pathway. Invivo experiments showed that EPCs seeded on PU-Au coated catheters effectively reduced thrombosis by differentiation into endothelial cells. Surface endothelialization with CD31 and CD34 expression as well as intimal formation with α-SMA expression was significantly accelerated in the group receiving EPC-seeded PU-Au catheters. Moreover, the analysis of collagen deposition revealed a reduction of fibrosis in the group receiving EPC-seeded PU-Au catheters as compared to the other groups. These results suggest that EPCs engineered with a proper elastic substrate may provide unique endothelialization and antithrombogenic properties that benefit vascular tissue regeneration. © 2014 Elsevier Ltd.
SDGs
Type
journal article