Autonomous screening of C. elegans identifies genes implicated in synaptogenesis
Journal
Nature Methods
Journal Volume
9
Journal Issue
10
Pages
977-980
Date Issued
2012
Author(s)
Abstract
Morphometric studies in multicellular organisms are generally performed manually because of the complexity of multidimensional features and lack of appropriate tools for handling these organisms. Here we present an integrated system that identifies and sorts Caenorhabditis elegans mutants with altered subcellular traits in real time without human intervention. We performed self-directed screens 100 times faster than manual screens and identified both genes and phenotypic classes involved in synapse formation. © 2012 Nature America, Inc. All rights reserved.
Type
journal article