Cancer Cell Uptake Behavior of Au Nanoring and Its Localized Surface Plasmon Resonance Induced Cell Inactivation Efficiency
Date Issued
2014
Date
2014
Author(s)
Chu, Che-Kuan
Abstract
Au nanorings (NRIs), which have the LSP resonance wavelength around 1058 nm, either with linked antibody (NRI-AB) or without antibody (NRI-control), are applied to SAS oral cancer cell for cell inactivation through the localized surface plasmon (LSP)-induced photothermal effect when it is illuminated by a laser of 1065 nm in wavelength. Various incubation times after the application of Au NRIs to cell are considered for observing the variations of cell uptake of Au NRI and hence the threshold laser intensity for cancer cell inactivation. In each case of incubation time, the SAS cell is washed for evaluating the Au NRI number per cell adsorbed and internalized by the cells. Also, the Au NRIs adsorbed (remaining on cell membrane) are etching with KI/I2 to evaluate the Au NRI number per cell internalized by the cells. The threshold laser intensities before washout, after washout, and after KI/I2 etching are calibrated from the circular area sizes of inactivated cells around the illuminated laser spot center under the illuminations of various laser power levels. The adsorbed and internalized Au NRIs per cell are obtained from inductively coupled plasma mass spectrometry measurements of the flushed solution in the KI/I2 etching process and the remaining cell solution, respectively. By using Au NRIs with antibody, it is found that the internalized Au NRI number per cell increases with incubation time. However, the adsorbed Au NRI number per cell reaches a maximum at 12 hrs in incubation time. The cell uptake behaviors of Au NRIs without antibody are similar to those with antibody. Nevertheless, the NRI numbers per cell are significantly smaller. Meanwhile, the incubation time for the maximum adsorbed NRI number per cell is delayed to 20 hrs. By comparing the variation of threshold laser intensity for cell inactivation, it is found that the adsorbed NRIs can cause more effective cancer cell inactivation, when compared with the internalized NRIs. The minimum threshold laser intensities after cell solution washout in either case with or without antibody are observed before KI/I2 etching under the aforementioned incubation time conditions of maximum adsorbed NRI number per cell.
Subjects
奈米環
細胞滅活
SDGs
Type
thesis
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