Lidocaine與Methylprednisolone對於大鼠視網膜缺血再灌流病變模式的神經保護效力
The Neuroprotective Effects of Lidocaine and Methylprednisolone in a Rat Model of Retinal Ischemia-Reperfusion Injury
Date Issued
2006
Date
2006
Author(s)
Tsai, Wen-Chih
DOI
zh-TW
Abstract
Purpose: To evaluate the neuroprotective effects of intravenous (IV) lidocaine (LDC) and methylprednisolone (MP) against the retinal ischemia-reperfusion (IR) insult. Methods: SD rats were divided into 3 groups, the IR control (n=5), LDC (n=7) and MP (n=7). Saline was infused into the anterior chamber using 30-gauge needle, which created a high intraocular pressure than blood pressure and lasted for 45 min. LDC bolus (1.5mg/kg) was IV injected 30 min before ischemia and then a constant rate infusion (CRI) with 2mg/kg/hr was given until 60 min after reperfusion. MP bolus (30mg/kg) was IV administered twice at 2 min before and immediately after ischemia, respectively. The damage to retinal function was evaluated by electroretinogram (ERG) 3 days after ischemia and by morphometrical histology analysis 7 days after ischemia. Results: The functional analysis of the retina by ERG revealed a 35.2% reduction of a-wave in the IR control group, 49.7% reduction in the LDC group but no significant change in the MP group. The b-wave reduction showed 81.0% in the IR control, 80.7% in the LDC group and 17.6% in the MP group. In the morphometrical histology, the retinal inner plexiform layer/outer nuclear layer (IPL/ONL) ratio was reduced following IR damage compared with that in the normal control. The IPL/ONL ratio was reduced to 48.8% in the IR control group, 80.1% in the LDC group and 96.2% in MP group. Conclusions: The MP showed significantly good neuroprotective effects on retinal IR injury, and the LDC showed moderate neuroprotective effects demonstrated in histology but not in retinal function.
Subjects
缺血再灌流病變
利多卡因
命得生
大鼠
視網膜
ischemia-reperfusion injury
lidocaine
methylprednisolone
rat
retina
Type
thesis
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