從肝細胞癌病人產生樹突狀細胞及其分析
Other Title
Generation and functional characterization of dendritic cells from
patients with hepatocellular carcinomas
patients with hepatocellular carcinomas
Date Issued
2002
Date
2002
Author(s)
陳健弘
DOI
902314B002202MH
Abstract
Since 1984, hepatocellular carcinoma
(HCC) has been the leading cause of cancer
death in Taiwan. Though regular sonographic
examination can early detect small HCC and
there are many therapeutic modalities for
HCC, the therapeutic results remains
unsatisfactory. In this regard, immunotherapy
is an attractive approach. The potential
attractiveness of using immunotherapy for
HCC treatment is further strengthened by the
observation that HCC with marked
inflammatory cell infiltration has less portal
vein invasion, less recurrent rate and better
5-year survival compared with HCC without
lymphocyte infiltration.
Dendritic cells (DCs) are the most
potent professional antigen-presenting cells
that prime helper and killer T cells in vivo. However, several reports described that there
may be some defects in the DCs of cancer
patients. Dysfunctional DC will have a
tremendous influence on the immune
responses. Dysfunctions of DCs in cancer
patients include reduced DC numbers in the
peripheral blood of cancer patients,
prevalence of immature phenotype, reduced
level of interleukin-12 and increased
interleukin-10 production, reduced repression
levels of CD86 or CD80, and increased
apoptosis.
Understanding of the DC biology in
HCC patients is the prerequisite for the future
successful application of DC-based
immunotherapy. Around 60-80% of HCCs in
Taiwan were related to HBV infection and
HBV or HCV per se may have different
influence on the DC function. Therefore, in
this current project, we focused on
HBV-related HCCs.
We generated DCs from peripheral
blood of liver cirrhosis and HCC patients.
We found that both the immature and mature
DCs generated from HCC patients showed
similar immunophenotypes as the DCs
generated from normal controls and cirrhotic
patients. In addition, the DCs from HCCs did
not lose their functions as assayed by
endocytosis capability and mixed lymphocyte
reactions. Our data suggested that DCs
generated from PBMCs of HCCs could be
good vehicles to be used in the DC-based
immunotherapy.
Subjects
Hepatocellular carcinoma
dendritic cell
peripheral blood
immune
dysfunction
dysfunction
phenotypes
SDGs
Publisher
臺北市：國立臺灣大學醫學院內科
Type
report
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