Improvement of Helicobacter pylori-associated peptic ulcer by Nano-emulsified camellia oil
Date Issued
2015
Date
2015
Author(s)
Hsieh, Yu-Chun
Abstract
Camellia oil (Camellia oleifera Abel.) contains abundance of polyunsaturated fatty acid (PUFA) such as oleic acid and linoleic acid. In traditional Chinese medicine, people believe camellia oil can effectively protect human gastrointestinal system such as stomach disease. However, the effect of camellia oil on H. pylori-associated peptic ulcer remains unknown. Therefore, the aim of this study was to investigate whether the camellia oil can inhibit the growth of H. pylori and thus improve peptic ulcer. There were three points of this study. The first part was to investigate how to prepare the nano-emulsified camellia oil. And then we examined if the nano-emulsified camellia oil would inhibit the growth of H. pylori. Finally we co-cultured H. pylori with gastric epithelial cells (AGS cell) or THP-1 cell and investigate the anti-inflammatory effect of nano-emulsified camellia oil. The nano-emulsified camellia oil was formulated by ultrasonication and homogenization, and then emulsifiers with different HLB values were added. Result showed the size of prepared nano-emulsified camellia oil was 142.6 ± 1.3 nm, and the nano-emulsified camellia oil could inhibit the growth of H. pylori in a dose dependent manner, from 0.025% to 0.25%. In the IL-8 secretion test, we found that treatment with nano-emulsified camellia oil decreased IL-8 secretion in AGS and THP-1 cell model. This investigation could better help us understand the effect of camellia oil on H. pylori-associated peptic ulcer disease, and elucidate the possible mechanism. At last, we expect that camellia oil could be a useful adjunct in H. pylori eradication treatment.
Subjects
nano-emulsion camellia oil
Helicobacter pylori
peptic ulcer disease
Interleukin-8
Gastric epithelial cells
SDGs
Type
thesis
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