Solvatochromic Fluorescence of a GFP Chromophore-Containing Organogelator in Solutions and Organogels

Solvatofluorochromism, a solvation effect on the fluorescence color of an organic dye, is a property generally limited to fluid solutions. We demonstrate herein the concept of solid-state solvatofluorochromism by using an organogelator (1-SG), which consists of a solvatofluorochromic green fluorescence protein (GFP) chromophore (1) and a sugar gelator (SG). While 1-SG could be located in the liquid phase or in the fibrous solid matrix of the SG gel, our results show that the one in the solid matrix but near the liquid interface has superior fluorescence stability and quantum efficiency as well as solvatofluorochromicity than the one in the liquid phase. In addition, the phenomenon of fluorescence turn-on occurs when the gel is formed in protic solvents. These features have been applied to perform multicolor fluorescence patterning, chemical vapor sensing, data encryption and decryption, and real-time fluorescence cell imaging. ?