The combined effects of probiotics and nutritional supplement containing fish oil on gut immune response and gut-brain axis
Date Issued
2014
Date
2014
Author(s)
Huang, Sheng-Fu
Abstract
Day and night, gut immune system encounters numerous foreign antigens. As a primary site of immune response initiation, it is important for gut immune system to distinguish from self and non-self antigen and affect subsequent immune response properly. The target product of this research includes two popular dietary factor: probiotics and DHA. It has been shown that both of them have certain immunomodulatory and anti-inflammatory effects. Here we give the treatment of probiotics and nutritional supplement containing DHA, separately or combined, to clarify their effect on gut immune system. In addition, we will measure the serotonin content in both intestine and brain to elucidate the link between probiotics or DHA, serotonin and gut-brain axis preliminarily.
In non-specific immune animal model, 6 week-old female BALB/c mice were divided into several groups randomly: Ctrl, Probio, Suppl, (P+S)-0.5x, (P+S)-1 xand (P+S)-5x. Animal were sacrificed after 6-week treatment. In OVA-specific immune model, 6 week-old female BALB/c mice were divided into groups as previous. The treat dosage of (P+S)-5 xgroup was adjusted to (P-S)-2x, which received 2X dosage. Mice were immunized by OVA Intraperitoneal injection and sacrificed after 9-week sample treatment. After sacrifice, we analyzed the cytokine production of Peyer’s Patch (PP) and mesenteric lymph node (MLN) primary cell culture and the immunomodulatory cell populations in PP, MLN and splenocyte (SPL). we additionally measured the serotonin content in the tissue homogenate of intestine and brain via ELISA.
Although the percentages of regulatory cell population were not affected, combined or separate sample treatment of probiotics and nutrition supplement containing DHA can increase regulatory cytokine production, including IL-10 and TGFβ. This data implies that the product has certain immunoregulatory function. On the other hand, probiotics and nutrition supplement containing DHA also made some impacts on the serotonin content in intestine and brain. However, the precise effects and mechanisms of these two dietary components on gut-brain axis need further elucidations.
Subjects
益生菌
腸道免疫
樹突細胞
調節型T細胞
腸-腦軸
血清素
Type
thesis
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