Sensitivity Improvement of Sulfonamides Analysis in Micellar Electrokinetic Chromatography/Electrospray Ionization Mass Spectrometry
Date Issued
2008
Date
2008
Author(s)
Liu, Yi-Lin
Abstract
To reduce the introduction of nonvolatile surfactants into the ESI source, a low flow-liquid junction interface was utilized in the MEKC-ESI/MS analysis of nine sulfonamides. Under pH 5.5 while the micelles would flow toward the inlet reservoir, the micelle in the junction could flow into the column, thus the separation efficiency could be maintained. However, separation of only seven sulfonamides could be observed. Since two sulfonamides whose binding forces with micelles are so strong that they could not be detected. Changing pH to 6.2, the EOF is faster than the mobility of micelle, thus all the nine sulfonamides could be detected. To avoid the micelle flow to the ESI source, organic solvent was added to the junction and the connecting capillary. With this arrangement, the EOF was reduced and the micelles would flow into the junction. In Comparison with the analyses using a low flow interface, this method provides better sensitivity.
Subjects
MEKC-ESI/MS
signal suppression
sulfonamides
liquid junction interface
low flow interface.
Type
thesis
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