Antcins, triterpenoids from Antrodia cinnamomea, as new agonists for peroxisome proliferator-activated receptor α
Journal
Journal of Food and Drug Analysis
Journal Volume
27
Journal Issue
1
Pages
295-304
Date Issued
2019
Author(s)
Abstract
Peroxisome proliferator-activated receptor α (PPARα) is a nuclear hormone receptor that transcriptionally regulates lipid metabolism and inflammation; therefore, PPARα agonists are promising agents to treat dyslipidemia and metabolic disorders. PPARα full agonists, such as fibrates, are effective anti-hypertriglyceride agents, but their use is limited by adverse side effects. Hence, the aim of this study was to identify small molecules that can activate PPARα while minimizing the adverse effects. Antrodia cinnamomea, a rare medical mushroom, has been used widely in Asian countries for the treatment of various diseases, including liver diseases. Antcin B, H and K (antcins) and ergostatrien-3β-ol (EK100) are bioactive compounds isolated from A. cinnamomea with anti-inflammatory actions. Antcins, ergostane-type triterpenoids, contain the polar head with carboxylate group and the sterol-based body. Here, we showed at the first time that sterol-based compounds, antcins, but not EK100, activate PPARα in a cell-based transactivation study. The in silico docking studies presented several significant molecular interactions of antcins, including Tyr314, and His440 in the ligand-binding domain of PPARα and these interactions are required for helix 12 (H12) stabilization. We propose that PPARα activation activity of antcins is related to their binding mode which requires conventional H12 stabilization, and that antcins can be developed as safe selective PPARα modulators. ? 2018
Other Subjects
antcin b; antcin derivative; antcin h; antcin k; cell nucleus receptor; ek 100; histidine; peroxisome proliferator activated receptor alpha; peroxisome proliferator activated receptor alpha agonist; peroxisome proliferator activated receptor gamma; pirinixic acid; triterpenoid; tyrosine; unclassified drug; antcin B; antcin K; cholest 4 en 3 one; cholestane derivative; peroxisome proliferator activated receptor alpha; plant extract; triterpene; animal cell; antiinflammatory activity; Antrodia camphorata; Article; controlled study; female; ligand binding; molecular docking; molecular interaction; nonhuman; transactivation assay; Antrodia; chemistry; human; metabolism; Antrodia; Cholestenes; Cholestenones; Humans; Molecular Docking Simulation; Plant Extracts; PPAR alpha; Triterpenes
Publisher
Elsevier Taiwan LLC
Type
journal article