Distinct trajectory of gut microbiota driven by a human oral commensal: insights from a murine study.
Journal
Journal of oral microbiology
Journal Volume
17
Journal Issue
1
Start Page
Article number 2569524
ISSN
2000-2297
Date Issued
2025
Author(s)
Lin, Wei-Ting
Lee, Shiao-Pieng
Li, Chin
Chang, Chia-Bin
Chien, Hsiu-Chuan
Wu, Shu-Fen
Tseng, Yu-Chao
Abstract
Background: Oral microbes modulate the gut microbiota. Haemophilus parainfluenzae, a core human oral commensal with immunomodulatory properties, is reduced in autoimmune diseases, while mitigating Sjögren's syndrome-like disease with improved oral microbiota in female NOD mice. However, whether it modulates the gut microbiota remains unknown. Objective: To study the modulatory effect of oral H. parainfluenzae inoculation on the gut microbiota. Design: Female NOD mice were orally inoculated with H. parainfluenzae following antibiotic treatment. Fecal samples were collected pre- and post-inoculation for 16S rRNA gene sequencing. Splenic antigen-presenting cells were analyzed for systemic immunomodulation. Results: Despite prominent convergence of diversity and beta dissimilarity within each group, H. parainfluenzae led to distinct core microbiota and overall microbial community. While reducing the Firmicutes-to-Bacteroidetes ratio, H. parainfluenzae enriched Bacteroidaceae and its genus Bacteroides. Bacteroides acidifaciens, a beneficial gut commensal, was enriched in ASV-level analyses. The splenic dendritic cells were reduced. Notably, neither did H. parainfluenzae establish ectopic gut colonization, nor was sustained oral colonization required, indicating that non-viable microbes may be sufficient to direct these responses. Conclusions: H. parainfluenzae drives a distinct gut microbiota reconstitution trajectory, characterized by B. acidifaciens enrichment without establishing notable colonizations, supporting its role in the oral-gut axis and warranting future postbiotic research.
Subjects
Bacteroides acidifaciens
Haemophilus parainfluenzae
NOD mice
Oral-gut axis
ectopic colonization
immunomodulation
post-antibiotic reconstitution
postbiotic
SDGs
Type
journal article