The Study of neuron culture on polylysine-like materials
Date Issued
2009
Date
2009
Author(s)
Wang, Yu-Shin
Abstract
Poly-D-lysine is a material that usually used in cell culture, especially to be deal as control material for other materials test in neural culture. The NH3+ group on poly-D-lysine would be positive charge at pH=7.4 which is a common conditions in cell culture, and it will be helpful for neuron adherence on material due to the overall negative charge on the cell surface.he study is to determine the different behaviors of neuron cultured on several materials which have similar structures to the poly-D-lysine by using in vitro neuron culture. We wish to find new materials that can be substitutes for poly-D-lysine. Besides, we want to know which part of structure of poly-D-lysine is useful for neural culture, and finally to create new materials that cheaper and more effective than poly-D-lysine on neural culture.he behaviors of neurons are determined by LDH and MTT first, and the morphology is checked by using microscope including phase contract and fluorescence. The expression of functional proteins is checked by immunobloting. And finally, the ability of transmission is determined by measurement of the glutamate release and phosphor-synapsin I(Ser603).n this study, the structure have four carbon on the residue which is similar to poly-D-lysine is the best one in the numbers of cerebellar granule cells attached on material. But if we adjust the concentration of coating, neurons can have similar expression on almost all materials.nd we also found that the low concentration of coating will made neurons have a little slide to form a big network without neurites broken. Besides, if we divide the among of functional expression with cell number, the average behaviors of big-network forms are not worse than single-adherent forms.lthough neurons grow in vivo are not always at single-attachment forms, the big-network forms caused by low-concentration-coating might be a new model for neuron culture.
Subjects
cerebellar granule neurons
poly-D-lysine
glutamate release
functional group
big-network form
Type
thesis
File(s)![Thumbnail Image]()
Loading...
Name
ntu-98-R95548054-1.pdf
Size
23.53 KB
Format
Adobe PDF
Checksum
(MD5):5db9d80e29d2bef45971a2eb3bda83b6