Progranulin is Required for Liver Regeneration in The Partial Hepatectomized Zebrafish
Date Issued
2012
Date
2012
Author(s)
Chiang, Keng-Yu
Abstract
Physiologic liver growth not only occurs in embryonic development, but also in the liver regeneration process that was activated after liver injury. According to our previous research, it indicated that progranulin could modulate MET, the receptor of hepatocyte growth factor, signaling to regulate liver growth. Progranulin is a multifunctional secreted glycoprotein. It has been known that plays a critical role in cell growth, wound healing, tumorigenesis, inflammation response, neurodevelopment, and neurodegeneration. This research would study the regulatory role of progranulin that involve in liver regeneration. We established a liver regeneration model after partial
hepatectomy in liver-specific fluorescent adult zebrafish. The result of quantitative-PCR showed that mRNA expression of progranulin, cell cyele and proliferation related genes
were induced after partial hepatectomy. To study the functional progranulin A in liver regeneration. We use the loss of function and gain of function assay. In our grnA
morphant showed a weak recovery with delayed cell cycle related gene and suppressed cell proliferation related gene that specifically at 36 hours after partial heapatectomy.
On the other side, in our liver specific grnA over expression transgenic zebrafish showed a faster recovery than wild-type zebrafish after partial hepatectomy. In
conclusion, grnA mediate met signaling is required for liver regeneration.
Subjects
Zebrafish
Progranulin
Met
Partial Hepatectomy
Liver Regeneration
Type
thesis
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