Polyunsaturated fatty acids production with a solid-state column reactor
Resource
Bioresource Technology 99 (14): 6181-6189
Journal
Bioresource Technology
Pages
6181-6189
Date Issued
2008
Date
2008
Author(s)
Jang, Hung-Der
Yang, Shang-Shyng
Abstract
To investigate the potential production of polyunsaturated fatty acids (PUFAs), a solid-state column reactor of rice bran with Mortierella alpina was used. The optimal conditions for PUFAs production were rice bran supplementation with 3.75% (w w-1) nitrogen source at initial moisture content 57%, initial pH 6-7, aeration, and incubation at 20 °C for 5 days and then at 12 °C for 7 days. Each gram of substrate carbon yielded 127 mg of total PUFAs, 12 mg of eicosapentaenoic acid (EPA), 6 mg of arachidonic acid (AA), 5 mg of α-linolenic acid (ALA), and 117 mg of linoleic acid (LA) after 12 days incubation. Aeration enhanced the productions of AA, EPA, and total PUFAs. Supplementation of the nitrogen source on the fourth day and then a shift to lower temperature on the fifth day increased EPA production. © 2007 Elsevier Ltd. All rights reserved.
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