Polyglycolic acid/chitosan glue and apoptosis of endometriotic cells
Resource
Fertility and Sterility 84 (1): 75-81
Journal
Fertility and Sterility
Journal Volume
84
Journal Issue
1
Pages
75-81
Date Issued
2005
Date
2005
Author(s)
Abstract
Objective: To induce apoptosis of endometriotic cells of patients with endometriosis. Design: To demonstrate that polyglycolic acid/chitosan glue directly inhibits cell proliferation by inducing apoptosis. Setting: University hospital infertility center. Patient(s): Twelve women who visited the center for infertility therapy. Intervention(s): Polyglycolic acid/chitosan glue was applied into primary endometriotic cells; the manipulated cells were collected 1-4 days after polyglycolic acid/chitosan glue treatment. Main Outcome Measure(s): Primary endometriotic cell cultures from eutopic endometriotic tissue were established. The effect of the novel biological glue, polyglycolic acid/chitosan glue A, on endometrial cells in vitro was examined. The different stages of apoptosis were analyzed using flow cytometry with fluorescein isothiocyanate conjugate (FITC)-annexin V and propidium iodide staining. Result(s): The growth inhibitory effects of polyglycolic acid/chitosan glue A on endometrial cells were found to be dose-response and time dependent. Less than 15% viability was detected in cultures containing 2,000 μg of polyglycolic acid/chitosan glue A after 4 days of treatment. Induced apoptosis and caspase activity were revealed. The caspase-3 activity increased 2.2-fold with 4 days of culture with 2,000 μg of polyglycolic acid/chitosan glue A. Conclusion(s): This is the first study to demonstrate that polyglycolic acid/chitosan glue directly inhibits cell proliferation by inducing apoptosis, thus suggesting that this new biological glue may be useful for endometriosis therapy. ?2005 by American Society for Reproductive Medicine.
Subjects
Apoptosis; Chitosan; Endometriosis; Polyglycolic acid
SDGs
Other Subjects
caspase 3; chitosan; fluorescein isothiocyanate; glue; lipocortin 5; polyglycolic acid; propidium iodide; apoptosis; article; cell culture; cell proliferation; cell viability; clinical examination; conjugate; controlled study; dose response; endometriosis; endometrium cell; enzyme activity; female; flow cytometry; human; human cell; human tissue; infertility therapy; priority journal; staining; time; university hospital; Apoptosis; Cell Proliferation; Cells, Cultured; Chitosan; Dose-Response Relationship, Drug; Endometriosis; Endometrium; Female; Humans; Polyglycolic Acid
Type
journal article
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