Development of a Thin Film Polymerase Chain Reaction and Fluorescence Detection System
Date Issued
2005
Date
2005
Author(s)
He, Guo-Cheng
DOI
zh-TW
Abstract
A thin-film Polymerase Chain Reaction (TF-PCR) system and a fluorescence detection system have been developed. Made of PDMS, the TF-PCR is fabricated by casting with easy of manufacturing and very high quality. The TF-PCR contains thermoelectric cooling components (TEC) as heaters, and uses a centrifuge system fan for forced convention cooling. The temperature controlling system is composed of a thermocouple as a temperature sensor, a DAQ card gather to voltage signals and a PC with the LabVIEW program. The monitoring and controlling of the TEC heater is through a PID program. Successful amplification of DNA strains of a shrimp virus with 120 and 226 base pair has been demonstrated by this TF-PCR. The fluorescence detection system can be divided into two subsystems. The LASER-induced fluorescence detecting subsystem includes a Green color semiconductor LASER (532nm in wavelength) as an exciting light source, followed by proper optical components and a photo multiplying tube (PMT), then fluorescence signals from DNA samples can be caught. The fluorescence image subsystem has a UV light source and can extract images from a CCD camera. This fluorescence detection system also uses step motors to control the sample stand, so that fixed scanning or moved scanning is possible. This fluorescence detection system is a multiple functional detecting system.
Subjects
聚合酵素連鎖反應:螢光檢測:凝膠電泳
Polymerase chain reaction (PCR):fluorescence detection: gel electrophoresis
Type
thesis
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