Studies Of the FIN219 Mutation Resulting In Chilling Tolerance Increase By Proteomics and RT-PCR In Arabidopsis
Date Issued
2006
Date
2006
Author(s)
Pu, Fong-Iln
DOI
zh-TW
Abstract
Microarray data indicated that KIN2 gene, which encodes antifreeze protein, showed different expression level among Wild type (WT) and fin219 mutant in far-red light. To confirm microarray data,total RNA were extracted WT、FIN219 overexpressor(FIN219OE) and fin219 treated with far-red light and KIN2 expression were examined by RT-PCR. As well these different plants were treated with 4℃ for 3 months to test their tolerance to cold stress. Result indicated that fin219 mutant was healthier than FIN219OE and WT. To future understand the conditions expression levels of FIN219 gene and KIN2 gene under stress, a RT-PCR approach was used for this.The result revealed that the KIN2 expression was higher in fin219 mutant than WT and FIN219OE , which indicates that the fin219 mutant is more chilling tolerant.
The promoter sequence of the FIN219 was searched for cis-element revealed to the cold stress and found that C-repeat element and ABRE were present. To verify cold response,the FIN219 promoter::GUS transgenic plants were treated with cold and underwent for GUS-staining. The result indicated that FIN219 was also induced by cold. Proteomics tools helped to discover that 9-ciscaroteinoid dioxygenase(A4)、acetyl-CoA carboxylase/ ligase(A8)、ATEXLA2 (ARABIDOPSIS THALIANA EXPANSIN-LIKE A2)(A9)、putative AP2 domain containing protein RAP2.8(B2)、indole-3-glycerol-phosphate synthase(B6) have different expression patterns under various expression levels of the FIN219 gene and were involved in cold acclimation. Take together these data indicated that the fin219 mutant is more resistant to cold stresses than WT and FIN219OE plants.
Subjects
低溫
低溫抗性
蛋白質體學
chilling
chilling tolerance
proteomics
Type
other
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