飼糧二十二碳六烯酸對豬隻脂質代謝基因表現之影響(2/3)
Date Issued
2004
Date
2004
Author(s)
DOI
922313B002127
Abstract
飼糧中脂肪酸可被動物利用而貯存於
各種組織。許（2002）於飼糧中添加2％ 二
十二碳六烯酸DHA 油，顯著增加豬隻各組織
中DHA 之含量，並顯著抑制仔豬肝臟中與脂
質新生合成相關的轉錄因子Sterol Regulatory
Element-Binding Protein-1（SREBP-1） mRNA
的表現。本研究的目的在餵飼豬隻高DHA 或
高硬脂酸飼糧，測試不同脂肪酸組成對豬隻
基因表現的影響。結果發現飼糧中添加2％
DHA 油18 天，顯著增加豬隻各肝臟中DHA
之含量，並顯著增進豬隻肝臟中與脂質氧化
有關基因的表現。利用抑制性雜合扣除法發
現飼糧中添加DHA 可刺激一些與脂質代謝
相關之新穎基因的表現，本計畫亦初步利用
差異篩選法確認12 已知基因和3 個新的基因
表現隨著飼糧中不同的DHA 而有所不同。這
些已知基因包括：alcohol dehydrogenase、
dehydrogenase/reductase (SDR family)
member 8、serum amyloid protein、serine
protease inhibitor I、fibrinogen gamma
polypeptide、vitamin D binding protein、
α1-microglobulin-bikunin precursor 、
glutathione S- transferase、alpha-1 acid
glycoprotein 、X-box binding protein
pseudogene 1、H19 gene 和hypothetical
protein MGC56918。所以餵飼DHA 油除可生
產高DHA 肉品，亦可影響許多肝臟中基因的
表現。
Subjects
Dietary fatty acid composition can change
fatty acid profiles in several porcine tissues.
Hsu (2002) demonstrated 2% DHA oil in the
diet will significantly increased the DHA in
several porcine tissues. Such changes also
inhibited the expression of hepatic Sterol
Regulatory Element-Binding Protein-1
（ SREBP-1 ） mRNA . The purpose of
current project was to test the effect of dietary
high DHA and n-3 PUFA on FA composition
and gene expression in the liver. Dietary DHA
supplementation for 18 d increased the
expression of gene related to FA oxidation. The
suppression subtractive hybridization technique
was used to detect hepatic genes differentially
expressed in the DHA or tallow treated pigs.
A differential screening procedure confirmed
that there were 12 known genes and 3 novel
genes differentially expressed. They were
alcohol dehydrogenase, dehydrogenase/
reductase (SDR family) member 8, serum
amyloid protein, serine protease inhibitor I,
fibrinogen gamma polypeptide, vitamin D
binding protein, α1-microglobulin-bikunin
precursor, glutathione S-transferase, alpha-1
acid glycoprotein, X-box binding protein
pseudogene 1, H19 gene and hypothetical
protein MGC56918. Taken together, dietary
DHA treatment may be used to produce high
DHA meat products and to regulate gene
expression.
fatty acid profiles in several porcine tissues.
Hsu (2002) demonstrated 2% DHA oil in the
diet will significantly increased the DHA in
several porcine tissues. Such changes also
inhibited the expression of hepatic Sterol
Regulatory Element-Binding Protein-1
（ SREBP-1 ） mRNA . The purpose of
current project was to test the effect of dietary
high DHA and n-3 PUFA on FA composition
and gene expression in the liver. Dietary DHA
supplementation for 18 d increased the
expression of gene related to FA oxidation. The
suppression subtractive hybridization technique
was used to detect hepatic genes differentially
expressed in the DHA or tallow treated pigs.
A differential screening procedure confirmed
that there were 12 known genes and 3 novel
genes differentially expressed. They were
alcohol dehydrogenase, dehydrogenase/
reductase (SDR family) member 8, serum
amyloid protein, serine protease inhibitor I,
fibrinogen gamma polypeptide, vitamin D
binding protein, α1-microglobulin-bikunin
precursor, glutathione S-transferase, alpha-1
acid glycoprotein, X-box binding protein
pseudogene 1, H19 gene and hypothetical
protein MGC56918. Taken together, dietary
DHA treatment may be used to produce high
DHA meat products and to regulate gene
expression.
Publisher
臺北市：國立臺灣大學動物科學技術學系暨研究所
Type
report
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