Higher infection of dengue virus serotype 2 in human monocytes of patients with G6PD deficiency
Journal
PLoS ONE
Journal Volume
3
Journal Issue
2
Pages
e1557
Date Issued
2008
Author(s)
Abstract
The prevalence of glucose-6-phosphate dehydrogenase (G6PD) deficiency is high in Asia. An ex vivo study was conducted to elucidate the association of G6PD deficiency and dengue virus (DENC) infection when many Asian countries are hyperendemic. Human monocytes from peripheral mononuclear cells collected from 12 g6PD-deficients and 24 age-matched controls were infected with one of two DENV serotype 2 (DENV-2) strains-the New Guinea C strain (from a case of dengue fever) or the 16681 strain (from a case of dengue hemorrhagic fever) with a multiplicity of infection of 0.1. The infectivity of DENV-2 in human monocytes was analyzed by flow cytometry. Experimental results indicated that the monocytes of G6PD-deficient patients exhibited a greater levels of infection with DENV-2 New Guinea C strain than did those in healthy controls [mean±5D:33.6%±3.5 [27.2% - 39.2%) vs 20.3%±6.2 (8.0% - 30.4%), P<0.011. Similar observations were made of infection with the DENV-2 16681 strain [40.9%±3.9 (35.1% - 48.9%) vs 27.4%±7.1 (12.3% - 37.1%), P<0.01]. To our knowledge, this study demonstrates for the first time higher infection of human monocytes in G6PD patients with the dengue virus, which may be important in increasing epidemiological transmission and perhaps with the potential to develop more severe cases pathogenically. ? 2008 Chao et al.
SDGs
Other Subjects
adult; article; Asia; clinical article; controlled study; dengue; Dengue virus; disease association; endemic disease; ex vivo study; flow cytometry; glucose 6 phosphate dehydrogenase deficiency; human; human cell; male; monocyte; mononuclear cell; pathogenesis; serotype; virus infectivity; virus strain; virus transmission; case control study; cell culture; glucose 6 phosphate dehydrogenase deficiency; monocyte; pathogenicity; virology; Dengue virus; Case-Control Studies; Cells, Cultured; Dengue; Dengue Hemorrhagic Fever; Dengue Virus; Glucosephosphate Dehydrogenase Deficiency; Humans; Monocytes
Type
journal article