Modulation of blood compatibility of polysulfone by polyelectrolyte layer-by-layer modification
Date Issued
2007
Date
2007
Author(s)
Wu, Shu-Pin
DOI
zh-TW
Abstract
Surface modification of biomaterials by polyelectrolyte layer-by-layer (LBL) deposition is a newly emerging method to modulate biocompatibility for biomedical devices. LBL deposition can be applied to biomedical devices with complicated geometries, which make it an attracting method. In this study we applied this method to improve blood compatibility of polysulfone (PSF). PSF, after sulfonation, was coated with multilayer of poly(allylamine hydrochloride) (PAH) and poly(acrylic acid) (PAA) which was assembled at either pH 2.0 or pH 6.5 by LBL deposition to desired number of layers. The modified surfaces were characterized by atomic force microscope, contact angle measurement, XPS and FTIR. We found that platelet adhesion was low on the LBL surfaces which were prepared at pH 2.0, while platelet adhesion was increased with increasing number of layers when the LBL surfaces were prepared at pH 6.5. Platelet adhesion was correlated to the adsorption of fibrinogen, the most critical plasma protein mediating platelet adhesion. Furthermore, platelets activation was increased with the layer numbers. In conclusion, platelet adhesion and activation is modulated by the pH of deposition solution and the number of layers of polyelectrolyte multilayer.
Subjects
聚碸
聚電解質多層膜
血液相容性
纖維蛋白原
血小板
Polysulfone
polyelectrolyte multilayer
blood compatibility
fibrinogen
platelet
Type
thesis