Succinic acid fermentation with immobilized Actinobacillus succinogenes using hydrolysate of carbohydrate-rich microalgal biomass
Journal
Bioresource Technology
Journal Volume
342
Date Issued
2021
Author(s)
Abstract
This work aimed to study the efficiency of polyvinyl-alcohol-immobilized Actinobacillus succinogenes ATCC55618 for succinic acid (SA) production. Batch fermentation (pH 7, 45% CO2 gas at 0.04 vvm) using glucose (40 g L-1) resulted in SA titer, 26.7 g L-1; productivity, 3.33 g L-1h?1; yield, 0.621 g g?1. Fed-batch mode with cyclic extrication of SA from the medium markedly enhanced the yield to 0.699 g g?1 and concentration to 59.5 g L-1. Batch fermentation using sugars derived from Chlorella vulgaris ESP-31 without yeast extract gave a SA productivity, concentration, and yield of 1.82 g L-1h?1, 36.1 g L-1, and 0.720 g g?1, respectively. Furthermore, continuous fermentation (at 6 h HRT) with microalgal sugar improved the productivity and yield to 3.53 g L-1h?1 and 0.62 g g?1, respectively, which is comparable to those obtained by using glucose. This study reports the highest productivity for SA fermentation using microalgae-derived sugars. ? 2021 Elsevier Ltd
Subjects
Actinobacillus succinogenes
Cell immobilization
Fermentation
Microalgae
Polyvinyl alcohol
Algae
Glucose
Microorganisms
Polyvinyl alcohols
Productivity
Acid fermentation
Acid production
Batch fermentation
Batch modes
Chlorella vulgaris ESP-31
Fed batches
Microalgal biomass
Succinic acids
Yeast extracts
carbohydrate
carbon dioxide
glucose
polyvinyl alcohol
succinic acid
sugar
yeast extract
bacterium
biomass
carboxylic acid
fermentation
immobilization
microalga
Article
Chlorella vulgaris
concentration (parameter)
continuous fermentation
controlled study
fed batch fermentation
hydrolysis
immobilized cell
nonhuman
Actinobacillus
Biomass
Carbohydrates
Succinic Acid
SDGs
Type
journal article