A Screen for modifiers of Atg1-mediated signaling in Drosophila development
Date Issued
2010
Date
2010
Author(s)
Chuang, Li-Jin
Abstract
Abstracts
Autophagy is a highly conserved cellular process that involves vesicle-mediated sequestration and degradation of cytoplasmic proteins and organelles. Atg1 is a Ser/Thr kinase that is regulated by TOR-dependent signaling. In yeast, studies have found the requirement for Atg1 kinase activity in both CVT and autophagy. Thus, Atg1 is representing a nodal point for controlling multiple steps in autophagic process in response to various stresses. I have examined that overexpression of Drosophila Atg1 in the developing compound eye triggers cell death and results in eye roughness. Although a number of proteins have been found to associate with Atg1, the identification of Atg1 substrates important for autophagy remains a difficult task. To identify novel genes involved in the Atg1-mediated pathway, I carried out a dominant modifier screen of the Atg1-induced rough eye phenotype using contiguous chromosomal deficiencies that represent more than 70% of the Drosophila genome. Of the 277 deficiencies tested, 26 were identified as suppressors of Atg1 signaling. I characterize a subset of autosomal regions that strongly interact with Atg1. Three novel genes will likely identify Atg1 regulators and should shed some light on how cells are regulated by the balance between cell survival and cell death. I am currently investigating whether these regulators have physical interaction with Atg1 and what is the biological function in cell death.
Subjects
Autophagy
Apoptosis
Drosophila
Atg1
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