A Study on Cell-based Biosensor for Cytotoxic Assay of Human Natural Killer Cells against Cancer Cells
Date Issued
2011
Date
2011
Author(s)
Lai, Fei-Lung
Abstract
Natural killer (NK) cell transplantation therapy has the potential to be an effective treatment for some cancers. A growing number of clinical trial investigations indicate that this therapeutic approach may provide a promising new treatment for leukemia. However, the alloreactivity of NK cells from different healthy donors has shown various recovery response levels. Little is still known about the cytotoxicity of NK cells in the context of cancer treatment or the interaction between donor NK cells and recipient cancer cells. The primary method used to measure NK cell cytotoxicity is flow cytometry, a large and often costly system that requires millions of NK cells for each assay. Patients are often faced with situations that require immediate action, and it can be difficult to find a donor to provide so many NK cells on such short notice. As a result, no appropriate assessment strategy has been developed for selection of multiple healthy donors prior to NK cell transplantation.
This study describes a novel cell-based biosensor for use with heterogeneous cell suspensions for cell docking, culturing, and cell–cell contact and interaction by microfluidic manipulation. Using this biosensor, we investigated cell activity and cytotoxicity through a real-time monitoring microscope system. The results demonstrate the feasibility of using the cell-based biosensor for cytotoxicity of NK cells against cancer cells, and show its potential applications in immune cell transplantation therapy.
Subjects
NK cell
leukemia
cytotoxicity
MEMS
biochip
SDGs
Type
thesis
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