Lipid Raft and Caveolin Polarization Guide Fibroblast Directional Migration in Electric Fields via Src

Galvanotaxis is a phenomenon where cell migration directed by electric fields (EFs). Previous studies revealed that the redistribution of lipid raft guides cell directional motility by regulating integrin or caveolin-1, and cells has opposite directionality between AC and DC. This study indicates that lipid raft can be polarized under in 50Hz of alternative current, and the redistribution of lipid raft can be different in different migration phenotype cells. Lipid raft and caveolin-1 polarization can guide cell directional migration in EF by redistribute Src polarization. PI3K also involved in the pathway by affecting Src polarization. These results clarified how lipid raft polarization change cell migration in EF in two aspects: the position lipid rafts distribute in different situations, and the downstream pathways.