Rat Pheochromocytoma PC12 Cells in Culture
Journal
Methods in Molecular Biology
Journal Volume
2565
Pages
3-15
ISBN
978-1-0716-2670-2
978-1-0716-2671-9
Date Issued
2023-01-01
Author(s)
Chen, Pin Chun
Abstract
PC12 cells serve as a secretory cell model, especially suitable for studying the molecular mechanisms underlying fusion pore kinetics in regulated exocytosis of dense-core vesicles (DCVs). In this chapter, we describe a series of PC12 cell culture procedures optimized for real-time functional assays such as single-vesicle amperometry. In addition, these conditions have been widely used for single-cell biochemical assays such as the proximity ligation assay with immunostaining.
Subjects
Dense-core vesicle | Fusion pore kinetics | Norepinephrine | PC12 cell culture | Proximity ligation assay with immunostaining | Rat pheochromocytoma | Regulated exocytosis | Single-cell biochemistry assay | Single-vesicle amperometry
Publisher
Humana Press Inc.
Type
book part