在豬隻心房顫動模式中功能性基因體的變化(1/3)
Other Title
Functional Genomic Studies in a Porcine Model of Atrial
Fibrillation
Fibrillation
Date Issued
2003
Date
2003
Author(s)
賴凌平
DOI
912314B002273
Abstract
There are two specific aims in the present
study:
1. To establish a porcine model of atrial
fibrillation.
2. To study the changes of atrial tissue from
a viewpoint of function genomics by2-D
PAGE and DNA microarray techniques.
This project is a three-year project.
During the period between August 2002 and
now, we have already accomplished the
followings
1. To establish a porcine model of atrial
fibrillation by rapid atrial pacing. The
atrial fibrillation was observed after rapid
pacing for 4 weeks and was incessant
until sacrifice.
2. We have established the techniques of
cDNA microarray to profile the gene
expression pattern in porcine atrial tissue.
3. We have successfully used the 2D-PAGE
techniques to separate protein spots in
atrial tissue. These protein spot include
both known and unknown protein spots.
In the future two years, we will
accomplish the following items
1. We will continue the porcine atrial
fibrillation model and produce more pigs
for mRNA and protein experiment. We
will also produce sham pigs by
implanting the pacemaker without
pacing.
2. We will use the cDNA microarray
technique to compare the difference
between the gene expression pattern
between atrial fibrillation and control
pigs.
3. We will use the 2D-PAGE technique to
compare the protein spot pattern between
atrial fibrillation and control pigs.
4. We will compare the mRNA change and
protein change. Significant findings will
be re-confirm by Northern and Western
blotting techniques
With the present study, we expect to
identify crucial changes in the development
of atrial fibrillation. This information will
contribute to future development of treatment for atrial fibrillation.
Subjects
atrial fibrillation
microarray
2D-PAGEl
Publisher
臺北市：國立臺灣大學醫學院內科
Type
report
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