Glycan arrays on aluminum-coated glass slides
Journal
Chemistry-an Asian Journal
Journal Volume
3
Journal Issue
8-9
Pages
1395-1405
Date Issued
2008
Author(s)
Tseng, Susan Y.
Wang, Cheng-Chi
Lin, Chin-Wei
Chen, Cheng-Lung
Yu, Wen-Yueh
Chen, Chung-Hsuan
Wu, Chung-Yi
Wong, Chi-Huey
Abstract
We have developed a novel method of immobilizing glycans onto aluminum-coated glass (ACG) slides for potential use in disease diagnosis and drug discovery. The quality of these sugar chips can be assessed by mass spectrometry and fluorescence measurements with high sensitivity. The unique properties of ACG slides include: 1) the metal oxide layer on the surface can be activated for grafting organic compounds such as modified oligosaccharides; 2) the surface remains electrically conductive, and the grafted oligosaccharides can be simultaneously characterized by mass spectrometry and carbohydrate-binding assay; and 3) the slides are more sensitive than transparent glass slides in binding analysis. To demonstrate this, we synthesized a model compound of mannose with a built-in photocleavable linker bound to the ACG slide surface. The molecular weight of the grafted mannose was identified by mass spectrometry, and the slide was subjected to biotinylated ConA binding followed by Cy3-tagged streptavidin detection. This method was further extended to the preparation of glycan arrays containing lactose and the cancer antigen Globo H. ? 2008 Wiley-VCH Verlag GmbH & Co. KGaA.
Subjects
Fluorescent probes; Glycan arrays; Mass spectrometry; Microarrays; Protein binding
SDGs
Other Subjects
aluminum; cross linking reagent; glass; mannose; polysaccharide; protein; article; chemical structure; chemistry; mass spectrometry; photochemistry; scanning electron microscopy; spectroscopy; surface property; Aluminum; Cross-Linking Reagents; Glass; Mannose; Mass Spectrometry; Microscopy, Electron, Scanning; Molecular Structure; Photochemistry; Polysaccharides; Proteins; Spectrum Analysis; Surface Properties
Type
journal article