In Situ Hybridization Demonstrates That Porcine Teschovirus Can Infect a Wider Variety of Tissue Cell Types That Has Not Been Revealed
Date Issued
2014
Date
2014
Author(s)
Chen, Ya-Mei
Abstract
Porcine teschoviruses (PTVs) are RNA viruses that are classified within the genus Teschovirus of the family Picornaviridae and cause non-suppurative meningoencephalitis and polioencephalomyelitis. In Taiwan, the PTV infection is the first or secondary swine viral diseases and usually isolated together with other common pathogens. To investigate PTV distribution, tissues were collected from 29 culled postweaning pigs, and the positive rate of PTV detection by nested RT-PCR was 96.7% (by heads). The most positive tissues were the intestines where ileum had the significantly highest detection rate, followed by lymphoid organs, cranial portion of the brain, and visceral organs. Statistics showed that nonsuppurative encephalitis in the caudal brain had an obvious correlation with PTV detection. To recognize the relation between lesions and PTV locations, in situ hybridization (ISH) was performed. PTV positive signals were presented in the nervous system, immune system, kidney, jejunum, ileum and colon. PTVs are able to infect a wider variety of cell types than what has been known. This study furthers our understanding on the pathogenicity and pathogenesis of PTV. The study establishes in situ hybridization technique for PTVs detection and confirms the PTV cell tropism.
Subjects
porcine teschovirus
ISH
in situ hybridization
SDGs
Type
thesis