INTRACELLULAR OXYGEN RESPONSE MONITORING WITHIN MICROFLUIDIC DEVICES USING WIDEFIELD FREQUENCY DOMAIN FLUORESCENCE LIFETIME IMAGING MICROSCOPY (FD-FLIM)
Journal
MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
ISBN
9781733419031
Date Issued
2021-01-01
Author(s)
Abstract
Oxygen affects cellular responses in different aspects, e.g., metabolic pathways and plasma membrane integrity. Accurate measurement of intracellular oxygen concentration becomes an essential task in biomedical research. Due to the advantages provided by the widefield technique, the image acquisition speed of the system is faster and the exposure time can be reduced significantly. Therefore, it is suitable to be applied for time-lapsed observation to study transient phenomena. Using a novel microfluidic device, we can control the microenvironmental environment and real-time monitor the intracellular oxygen responses. Through this measurement, the cellular response to rapid oxygen microenvironment variation can be observed.
Subjects
Fluorescence Lifetime Imaging Microscopy (FLIM) | Intracellular Oxygen Response | Microfluidic Devices | Oxygen Microenvironment
Type
conference paper