Simple and cost-effective enzymatic detection of cholesterol using flow injection analysis
Journal
Analytical Sciences
Journal Volume
36
Journal Issue
9
Pages
1119-1124
Date Issued
2020
Author(s)
Abstract
A flow-injection analytical (FIA) system was developed for the determination of cholesterol concentrations based on enzymatic reactions that occurred in a cholesterol oxidase (CHOx)-immobilized, fused-silica capillary followed by electrochemical detection. The production of hydrogen peroxide from cholesterol in an enzymatic reaction catalyzed by CHOx was subsequently oxidized electrochemically at an electrode. Our FIA system demonstrated its cost-effectiveness and utility at an applied potential of 0.6 V (vs. Ag/AgCl), a flow rate of 100 μL/min and, under optimal conditions, the resulting signal demonstrated a linear dynamic range from 50 μM to 1.0 mM with a limit of detection (LOD) of 12.4 μM, limit of quantification (LOQ) of 44.9 μM, and the coefficient of variation of 5.17%. In addition, validation of our proposed system using a reference HDL-cholesterol kit used for clinical diagnosis suggested our FIA system was comparable to commercial kits for the determination of the cholesterol incorporation amount in various aqueous liposomal suspensions. These good analytical features achieved by FIA could make the implementation of this methodology possible for on-line monitoring of cholesterol in various types of samples. © 2020 Japan Society for Analytical Chemistry.
Subjects
Cholesterol; Cholesterol oxidase; Enzyme; FIA; Flow injection analysis; Hydrogen peroxide; Oxidation
Other Subjects
Chemical detection; Cholesterol; Cost effectiveness; Diagnosis; Fused silica; Hydrogen production; Suspensions (fluids); Coefficient of variation; ELectrochemical detection; Flow injection analysis; Fused-silica capillaries; Limit of quantifications; Linear dynamic ranges; Liposomal suspension; Production of hydrogen; Cost benefit analysis; cholesterol; cholesterol oxidase; immobilized enzyme; liposome; silicon dioxide; chemistry; cost benefit analysis; economics; flow injection analysis; genetic procedures; limit of detection; metabolism; procedures; Biosensing Techniques; Cholesterol; Cholesterol Oxidase; Cost-Benefit Analysis; Enzymes, Immobilized; Flow Injection Analysis; Limit of Detection; Liposomes; Silicon Dioxide
Type
journal article