A comparative study of interfaces for microchip micellar electrokinetic chromatography-electrospray ionization mass spectrometry using the surfactant ammonium dodecyl sulfate
Resource
ELECTROPHORESIS, 32(23), 3385-3391
Journal
Electrophoresis
Pages
3385-3391
Date Issued
2011
Date
2011
Author(s)
Huang, Ju-Li
Li, Fu-An
Her, Guor-Rong
Abstract
Using ammonium dodecyl sulfate (ADS) as the surfactant, the response of three common interfaces in the direct coupling of microchip micellar electrokinetic chromatography with electrospray ionization mass spectrometry was studied. In the range of 10-40mM surfactant, a conventional sheath liquid interface provided poorer sensitivity than both sheathless interface and low-sheath-flow interface. At a surfactant concentration <20mM, a low-sheath-flow interface exhibited less sensitivity than a sheathless interface; however, it outperformed the sheathless interface above a concentration of 20mM. At a surfactant concentration above 20mM, signal reduction due to dilution of the analyte compensated by signal enhancement gained from a reduction in ion suppression effect. The difference in responses of the interfaces was mainly due to the dilution effect, whereas the effect of flow rate became an important factor when the difference in responses between the interfaces was not significant. The utility of the PMMA microchip MEKC/MS using a low-sheath-flow interface was demonstrated by the analysis of sulfonamides at a concentration of 40mM. The interday precision was in the range of 4.9-14.5%, and the LOD was in the range of 0.34-1.03ng/mL (MEKC/MS/MS). © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Type
journal article
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