Profile diversity of galacto-oligosaccharides from disaccharides to hexasaccharides by porous graphitic carbon liquid chromatography-orbitrap tandem mass spectrometry
Journal
Food Chemistry
Journal Volume
390
Date Issued
2022
Author(s)
Abstract
Galacto-oligosaccharides (GOS) are important prebiotic supplements for commercial nutraceutical food. The prebiotic efficacy of functional GOS is dependent on their chemical profile. Screening potential markers aids specifications and quality control of GOS materials. However, profiling analysis of GOS with a degree of polymerization (DP) ≥ 4 is still challenging. This study presents a porous graphitic carbon liquid chromatography-orbitrap tandem mass spectrometry-based method that characterized 58 linear and 10 branched GOS and detected 59 non-reducing GOS from DP2 to DP6. The results indicated that 15 major group components with DP2-DP5 accounted for more than 65% of total GOS content in GOS samples, while non-reducing GOS components accounted for only 2.8–7.6%. Substantial variations in components occurred in samples from different batches and sources. Structural and constitutive diversity were dominated by DP3-DP5. This method can help control the quality of GOS products and be used to investigate the structural and prebiotic-efficacy relationships. © 2022
Subjects
Galacto-oligosaccharides; Orbitrap tandem mass spectrometry; Porous graphitic carbon liquid chromatography; Profiling analysis; Structural characterization
SDGs
Other Subjects
Carbon; High pressure liquid chromatography; Liquids; Mass spectrometry; Oligosaccharides; Quality control; Galacto-oligosaccharides; Orbitrap; Orbitrap tandem mass spectrometry; Porous graphitic carbon; Porous graphitic carbon liquid chromatography; Prebiotics; Profiling analyse; Structural characterization; Tandem mass spectrometry; Tandem-mass spectrometry; Porous materials; carbon; disaccharide; galactose; graphite; oligosaccharide; prebiotic agent; chemistry; liquid chromatography; porosity; procedures; tandem mass spectrometry; Carbon; Chromatography, Liquid; Disaccharides; Galactose; Graphite; Oligosaccharides; Porosity; Prebiotics; Tandem Mass Spectrometry
Type
journal article