Common human coronavirus-induced cross-reactive salivary antibodies exhibit neutralizing activity and protective potential against SARS-CoV-2.
Journal
Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi
ISSN
1995-9133
Date Issued
2026
Author(s)
Abstract
Background: Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) still evolves and spreads worldwide. Mucosal immunity is the first line of defense against SARS-CoV-2 infections. We tested the hypothesis of whether salivary antibodies (sIgA or IgG) induced by common human coronaviruses (HCoVs) have cross-reactivities and neutralizing effects against SARS-CoV-2. Methods: Paired saliva and serum samples were collected from healthy young adults (n = 98) in 1993 and uninfected healthcare workers during the pandemic (n = 79). sIgA and IgG antibodies against SARS-CoV-2 and HCoV-229E were detected, respectively. Results: A moderate to strong positive correlation was found between levels of anti-SARS-CoV-2 and anti-HCoV-229E sIgA and IgG in saliva. Specific anti-S of SARS-CoV-2 IgA were found in 14 % of saliva, whereas neither anti-NP nor anti-S IgG were found in serum collected in the pre-pandemic era. Cross-reactivities of these sustained anti-HCoVs sIgA or IgG were enriched and confirmed by immunoblotting and also exhibited neutralizing activities against SARS-CoV-2 and its variants using Luminex-based assay. Furthermore, the incidence rate ratios of subsequent SARS-CoV-2 infection were significantly lower in the healthcare workers group with high salivary anti-HCoV-229E S1 sIgA. Conclusion: Our findings suggest that salivary anti-HCoV-229E S1 sIgA antibodies with cross-reactivities against SARS-CoV-2 S1 protein may pose protective potential against SARS-CoV-2.
Subjects
Cross-reactive IgA or IgG
Salivary anti-coronavirus antibodies
Severe acute respiratory syndrome coronavirus-2
Type
journal article