Rapid bacterial detection using a microfluidic system integrating membrane filtration and surface-enhanced Raman scattering
Journal
22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Journal Volume
3
Pages
1765-1768
Date Issued
2018
Author(s)
Abstract
A bacterial detection method has been developed using a microfluidic system integrating membrane filtration and surface-enhanced Raman scattering (SERS). Based on its highly-sensitive and label-free features, SERS has been reported for detecting various bacteria by analyzing their secreted metabolites. However, a prolonged (>16 hours) culturing time is usually required due to the low concentration of bacteria (1-100 CFU/mL) in patients’ biological samples. To address this problem and simplify the sample pre-treatment process, we demonstrate a microfluidic system with membrane filtration and SERS detection to achieve high throughput (~1 mL/min) bacterial enrichment and real-time, label-free bacterial detection. Copyright ? (2018) by Chemical and Biological Microsystems Society. All rights reserved.
Subjects
Bacterial detection; Membrane filtration; Surface-enhanced Raman scattering (SERS)
Other Subjects
Bacteria; Fluidic devices; Metabolites; Microfiltration; Microfluidics; Raman scattering; Bacterial detection; Biological samples; Low concentrations; Membrane filtrations; Micro fluidic system; Rapid bacterial detections; Sample pretreatment; Surface enhanced Raman Scattering (SERS); Surface scattering
Type
conference paper