雞濾泡顆粒細胞感染腸炎沙門氏菌後降低黃體激素生成的機制
Journal
臺灣獸醫學雜誌
Journal Volume
39卷
Journal Issue
4期
Pages
225-232
Date Issued
2013
Abstract
腸炎沙門氏菌具有感染雞隻卵巢細胞的能力並可能在臨床上造成產蛋率下降。雞隻卵巢顆粒細胞（cGCs）具有類固醇生成能力，能夠產生誘發排卵所必需的黃體激素，但同時卻也屬於腸炎沙門氏菌偏好感染的濾泡細胞之一。本研究使用已進入排序之各期濾泡cGCs，在離體培養環境中添加黃體生成素共同培養模擬活體中內泌素調控現象，以觀察腸炎沙門氏菌感染對於cGCs類固醇生成能力的影響。結果顯示cGCs被感染後，應是由於細胞內類固醇生成急性調節蛋白（StAR）基因表現量下降，使得可提供類固醇生成作用的膽固醇量減少，進而能在試驗中觀察到cGCs的黃體激素分泌量顯著降低，致使體內黃體激素濃度達到高峰所需的時間增加。這個機制造成排卵間距延長，在臨床上就可能發生產蛋遲緩與產蛋率下降。另外，也觀察到另一個黃體激素生成調控因子3β-HSD的mRNA表現量，在發育程度較低的濾泡之cGCs有降低的趨勢，可能也涉入黃體激素降低的機制之中。未來將會進一步針對發育程度較低的濾泡感染後3β-HSD的表現量與活性進行探究。
With the ability to invade chicken ovarian follicular cells, Salmonella Enteritidis (SE) may cause a clinical decrease in egg production. While the chicken granulosa cells (cGCs) is the preferred site for SE infection, cGCs also have the capability of steroidogenesis to produce progesterone which is essential for ovulation. In order to realize the alteration of steroidogenesis during the SE infection, cGCs at distinct stages of follicular maturity were used. To imitate the effects of the endocrine, luteinizing hormone was applied during co-incubation in the in vitro bacterial infection. The results showed a significant reduction in mRNA expression of steroidogenic acute regulatory proteins (StAR) and a significant reduction in progesterone production in cGCs during the infection. Both results proved that the reduction of StAR is associated with deceased progesterone synthesis and the gradual accumulation period of progesterone to peak is therefore extended. The extension may result in an increased time in ovulation, and further decreased egg production. In addition, a trend of decreased expression of 3β-HSD which is another genetic regulatory factor of progesterone synthesis, in cGCs from follicles of less maturity, was observed. This may play a role in decreased progesterone production. Further study is needed to investigate the alteration of expression level and enzyme activity of 3β-HSD in follicles of less maturity during the SE infection.
Subjects
腸炎沙門氏菌; 雞卵巢顆粒細胞; 黃體激素; 類固醇生成急性調節蛋白; Salmonella Enteritidis; Chicken granulosa cells; Progesterone; StAR; 3β-HSD;
Type
journal article