Dynamic change of surface microbiota with different environmental cleaning methods between two wards in a hospital
Journal
Applied Microbiology and Biotechnology
Journal Volume
101
Journal Issue
2
Pages
771-781
Date Issued
2017
Author(s)
Abstract
Terminal disinfection and daily cleaning have been performed in hospitals in Taiwan for many years to reduce the risks of healthcare-associated infections. However, the effectiveness of these cleaning approaches and dynamic changes of surface microbiota upon cleaning remain unclear. Here, we report the surface changes of bacterial communities with terminal disinfection and daily cleaning in a medical intensive care unit (MICU) and only terminal disinfection in a respiratory care center (RCC) using 16s ribosomal RNA (rRNA) metagenomics. A total of 36 samples, including 9 samples per sampling time, from each ward were analysed. The clinical isolates were recorded during the sampling time. A large amount of microbial diversity was detected, and human skin microbiota (HSM) was predominant in both wards. In addition, the colonization rate of the HSM in the MICU was higher than that in the RCC, especially for Moraxellaceae. A higher alpha-diversity (p?=?0.005519) and a lower UniFrac distance was shown in the RCC due to the lack of daily cleaning. Moreover, a significantly higher abundance among Acinetobacter sp., Streptococcus sp. and Pseudomonas sp. was shown in the RCC compared to the MICU using the paired t test. We concluded that cleaning changes might contribute to the difference in diversity between two wards. ? 2016, Springer-Verlag Berlin Heidelberg.
Subjects
16S rRNA metagenomics; Acinetobacter; Environmental cleaning methods; Healthcare-associated infection; Medical intensive care unit; Respiratory care centre
SDGs
Other Subjects
Bacteria; Cleaning; Health care; Hospitals; Intensive care units; RNA; 16S ribosomal RNA; Acinetobacters; Bacterial community; Environmental cleaning; Metagenomics; Microbial diversity; Respiratory care centre; Terminal disinfection; Disinfection; RNA 16S; bacterial DNA; ribosome DNA; abundance; bacterium; biotechnology; colonization; genomics; health care; health risk; hospital sector; infectious disease; microbial activity; respiratory disease; species diversity; Acinetobacter; Article; bacterium isolate; cleaning; controlled study; disinfection; medical intensive care unit; metagenomics; microbial colonization; microbial community; microbial diversity; microflora; Moraxellaceae; nonhuman; Pseudomonas; Respiratory Care Center; skin flora; Streptococcus; surface property; Taiwan; ward; bacterium; chemistry; classification; cluster analysis; disinfection; DNA sequence; genetics; hospital; hospital service; human; intensive care unit; isolation and purification; microbiology; phylogeny; procedures; Taiwan; Acinetobacter; Acinetobacter sp.; Bacteria (microorganisms); Moraxellaceae; Pseudomonas sp.; Streptococcus sp.; Bacteria; Cluster Analysis; Disinfection; DNA, Bacterial; DNA, Ribosomal; Environmental Microbiology; Hospitals; Housekeeping, Hospital; Humans; Intensive Care Units; Metagenomics; Phylogeny; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Taiwan
Publisher
Springer Verlag
Type
journal article