Capillary electrophoresis for rapid detection of Escherichia coli
Date Issued
2006
Date
2006
Author(s)
Wu, Shu-Fen
DOI
zh-TW
Abstract
The specific detection of Escherichia coli (E. coli) is essential for water quality control because its presence points directly to the presence of enteric disease-causing bacterial. We developed a rapid, easy and economical method for the analysis of bacterial lysates by capillary electrophoresis coupled with laser-induced fluorescence (CE-LIF). Addition of 0.01% poly(ethylene oxide) (PEO) (Mw 300k g/mol) to the sample matrix and applied a 0.2% sodium dodecyl sulfate (SDS) plug (1.3 cm) before injecting large-volume (ca. 1.0 μL) sample zone can improve stacking and separation efficiencies. After injection of the large-volume samples, the proteins migrate against the electroosmotic flow (EOF) and enter 1.7% PEO (Mw 4M g/mol) zone; this process causes them to slow down and stack at the boundary between the PEO and sample zones. As a result, the characteristic peaks of E. coli lysate were identified by injecting as few as 3 × 105 cells. Owing to growth rapidly and predominance in fecal contaminated water, we could detect and identify culturable E. coli cells in 50 mL of pond water in campus within one working day.
Subjects
大腸桿菌
指標微生物
毛細管電泳暨雷射誘導螢光偵測
線上濃縮
細菌溶解產物
Escherichia coli
indicator microorganism
capillary electrophoresis coupled with laser-induced fluorescence
on-line concentration
bacterial lysates
Type
thesis
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