The specificity and accuracy of 111In-hexavalent lactoside in estimating liver reserve and its threshold value for mortality in mice
Journal
Journal of Hepatology
Journal Volume
63
Journal Issue
2
Pages
370-377
Date Issued
2015
Author(s)
Abstract
Background & Aims: The asialoglycoprotein receptor on hepatocyte membranes recognizes the galactose residues of glycoproteins. We investigated the specificity, accuracy and threshold value of asialoglycoprotein receptor imaging for estimating liver reserve via scintigraphy using 111In-hexavalent lactoside in mouse models. Methods: 111In-hexavalent lactoside scintigraphy for asialoglycoprotein receptor imaging was performed on groups of normal mice, orthotopic SK-HEP-1-bearing mice, subcutaneous HepG2-bearing mice, mice with 20-80% partial hepatectomy and mice with acute hepatitis induced by acetaminophen. Liver reserve was measured by relative liver uptake and compared with normal mice. Asialoglycoprotein receptor blockade was performed via an in vivo asialofetuin competitive binding assay. Results: A total of 73.64 ± 7.11% of the injection dose accumulated in the normal liver tissue region, and radioactivity was barely detected in the hepatoma region. When asialoglycoprotein receptor was blocked using asialofetuin, less than 0.41 ± 0.04% of the injection dose was detected as background in the liver. Asialoglycoprotein receptor imaging data revealed a linear correlation between 111In-hexavalent lactoside binding and residual liver mass (R2 = 0.8548) in 20-80% of partially hepatectomized mice, demonstrating the accuracy of 111In-hexavalent lactoside imaging for measuring the functional liver mass. Asialoglycoprotein receptor imaging data in mice with liver failure induced using 600 mg/kg acetaminophen revealed 19-45% liver reserve relative to normal mice and a fatal threshold value of 25% liver reserve. Conclusion: The 111In-hexavalent lactoside imaging method appears to be a good, specific, visual and quantitative predictor of functional liver reserve. The diagnostic threshold for survival was at 25% liver reserve in mice. ? 2015 European Association for the Study of the Liver. Published by Elsevier B.V. All rights reserved.
Subjects
111In-hexavalent lactoside scintigraphy; Asialoglycoprotein receptor; Liver reserve
SDGs
Other Subjects
asialofetuin; asialoglycoprotein receptor; hexavalent lactoside in 111; indium 111; paracetamol; radiopharmaceutical agent; unclassified drug; glycoside; lactosides; acute hepatitis; animal experiment; animal model; Article; cancer mortality; competitive binding assay; controlled study; in vivo study; liver cell carcinoma; liver failure; liver function; liver reserve; liver scintiscanning; measurement accuracy; mouse; nonhuman; partial hepatectomy; priority journal; radioactivity; animal; Bagg albino mouse; biosynthesis; Carcinoma, Hepatocellular; computer assisted tomography; liver; Liver Neoplasms, Experimental; male; metabolism; mortality; pathology; single photon emission computer tomography; survival rate; Animals; Carcinoma, Hepatocellular; Glycosides; Liver; Liver Neoplasms, Experimental; Male; Mice; Mice, Inbred BALB C; Survival Rate; Tomography, Emission-Computed, Single-Photon; Tomography, X-Ray Computed
Publisher
Elsevier B.V.
Type
journal article