家禽腺病毒纖毛基因與毒力關係之研究
Date Issued
2001
Date
2001
Author(s)
DOI
892313B002187
Abstract
We designed a pair of primers based on the
sequence of penton of CELO fowl
adenovirus (FAV) for polymerase chain
reaction (PCR) and found that the PCR can
detect all 11 Taiwan strains. Another pair of
primers was also designed based on the fiber
gene sequence. Utilizing the primers
combined with the restriction enzyme profile,
the product of PCR from 9 of 11 Taiwan
strains were sequenced and compared with
each other and the published fiber sequence
of CFA3, CFA40, FAV-1A, FAV-10 and
EDSV. The data showed that the Taiwan
strains could be divided into 2 major groups.
The subgroup-1 in group I composes of all
Taiwan strains isolated from chickens and 2
Australia strains, CFA3 and CFA40.
Whereas mild pathogenic FAV-1A and
FAV-10 was grouped in the subgroup-2 in
group I. Strains from pigeons and ducks
have significant difference from the FAV
isolates from chickens and less different from
EDSV. Strains isolated from pigeons and
ducks was grouped in the same group, group
II. The result also indicate that fiber has some
relationship in pathogenecity to different
animals in addition to being responsible for
variations in virulence in the fowl
adenoviruses.
Subjects
Fowl Adenovirus
Fiber Gene Sequence
Polymerase Chain Reaction
Publisher
臺北市：國立臺灣大學獸醫學系暨研究所
Type
report
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