Synthesis and evaluation of a new fluorescent transglycosylase substrate: Lipid II-based molecule possessing a dansyl-C20 polyprenyl moiety
Journal
Organic Letters
Journal Volume
12
Journal Issue
7
Pages
1608-1611
Date Issued
2010
Author(s)
Abstract
(Figure Presented) The preparation of a novel fluorescent lipid II-based substrate for transglycosylases (TGases) is described. This substrate has characteristic structural features including a shorter lipid chain, a fluorophore tag at the end of the lipid chain rather than on the peptide chain, and no labeling with a radioactive atom. This fluorescent substrate is readily utilized In TGase activity assays to characterize TGases and also to evaluate the activities of TGase inhibitors. © 2010 American Chemical Society.
SDGs
Type
journal article