Genetic characterization of the late blight resistant locus qPh2.1 in tomato
Date Issued
2016
Date
2016
Author(s)
Liang, Yu-Ya
Abstract
Our previous QTL mapping study showed that the wild tomato accession L3708 (Solanum pimpinellifolium L.) harbors a new late blight resistant gene qPh2.1 on chromosome 2, in addition to the Ph-3 gene on chromosome 9. The aims of present study were as following: (1) Ph-3 fine mapping; (2) genetic characterization of the resistance of qPh2.1. In the current study, the qPh2.1 resistance to late blight disease was validated and characterized using isogenic lines derived from the previous F2:3 genetic mapping population which was generated using L3708 as the resistant parent. Our results indicated that the resistant allele of qPh2.1 was recessive to the susceptible allele. The qPh-3 gene was delimited in the region between 71,356,855 bp to 71,501,998 bp; and the qPh2.1 gene was delimited in the chromosomal region ahead of 28,042,265 bp on chromosome 2, according to the tomato SL 2.5 genomic reference sequences.
Subjects
Tomato
late blight
resistant gene
Type
thesis
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