乳癌之腫瘤浸潤淋巴細胞中T細胞之研究(1/2)
Date Issued
2004-07-31
Date
2004-07-31
Author(s)
張金堅
DOI
922314B002279
Abstract
Lymphoid infiltration in tumor tissues has been demonstrated a favorable sign for
prognosis of hosts in several malignant tumors1-5. Therefore, lymphocytes’ infiltration is
considered a result of tumor targeted, specific interactions rather than of an inflammatory
response. Most of the infiltrating cells are CD3+ T cells with a variable number of CD4+ and
CD8+. In most of the tumors, no B cells are found and natural killer cells constitute only a
small minority of Tumor infiltrating lymphocytes (TIL)6-10.
In human breast cancer , there was a significant reverse correlation between the
intensity of the T-cell infiltration and the clinical stages. In general, lymphocytes are found
more frequently and more abundantly in cancer than in its normal counterparts. Furthermore,
it is observed an increased CD4+/CD8+ ratio correlated with tumor’s size and lymph node
metastases 11. Studies in experimental animals have shown that the adoptive transfer of TIL is
50-100 times competent than LAK cell in mediating tumor regression12. Thus, TIL is a
potentially promising candidate for adoptive immunotherapy. TIL from primary breast
carcinomas can be propagated in large numbers in vitro with rIL2 while still retaining
autologous tumor specificity and MHC-restricted CTL activity 13.
In frist year of this project, we explored the distribution of immune cells in cancer ,
normal tissue and peripheral blood. The amount of mononuclear cells per mg of tissue in
breast cancer was more than in its normal counterpart( 4.15 ± 3.86 x 103 vs. 2.99 ± 3.78 x
103cell/mg, P=0.016). Comparing the lymphocytes isolated from PBMCs and TILs, the
median percentage on infiltrating natural killer (NK) cells and B cells was significantly lower
in TILs than in PBMCs (P < 0.001 in NK cells and in B cells). We also found that the median percentage of CD3+ T cells in TILs was higher than that in PBMCs (P <0.001). High ratio of
CD8+ T cell subpopulation was noted within gated autologous CD3+ TILs than PBMCs
(63.1%±14.3%, vs. 33.3%±12.6%, P <0.001). Low ratio of CD4+ T cell subpopulation was
noted within gated autologous CD3+ TILs than PBMCs (36.95%±14.29% vs. 66.68%±12.62%,
P <0.001). The CD4+/CD8+ratio were significantly reversed in TILs (0.66±0.41 vs. 2.33±0.96,
P <0.001), which was in accordance with our previous finding.
The distribution of natural killer (NK) cells and B cells was similar in NILs and TILs.
High ratio of CD8+ T cell (66.70%±14.19%, vs. 50.16%±14.02%, P =0.002) and low ratio of
CD4+ T cell (33.30%±14.19% vs. 49.84%±14.01%, P=0.002) was also noted within gated
autologous CD3+ TILs than NILs. The CD4/CD8 ratio were also significantly reversed in
TILs (0.55±0.33 vs. 1.22±0.52, P <0.001), which was in accordance with our previous finding
in cervical cancer.
Subjects
Breast cancer
Tumor infiltrating lymphocyte (TIL)
T lymphocyte
SDGs
Publisher
臺北市：國立臺灣大學醫學院外科
Type
report
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