Immunotherapy suppresses the production of monocyte chemotactic and activating factor and augments the production of IL-8 in children with asthma
Journal
Journal of Allergy and Clinical Immunology
Journal Volume
98
Journal Issue
3
Pages
580-587
Date Issued
1996
Author(s)
Abstract
Background: Histamine-releasing factor consists of a group of cytokines that can cause basophils or mast cells to release histamine. However, the composition of histamine-releasing factor remains undefined. Objective: This study was done to measure the concentrations, in plasma and mononuclear cell culture supernatants from children with asthma, of chemokines that are known to contribute to histamine-releasing factor activity. Results: Plasma and mononuclear cell culture supernatants were obtained from 25 children newly diagnosed with asthma, 25 good responders to immunotherapy, 23 poor responder, 25 patents with acute attacks, and 13 normal subjects. All the patient groups produced, spontaneously and after stimulation with phytohemagglutinin and mite allergen, greater amounts of monocyte chemotactic and activating factor, macrophage inflammatory protein-1α, and RANTES (β chemokines) and IL-8 and growth-related gene α (α chemokines) than did normal subjects. Successful immunotherapy resulted in decreased production, especially the spontaneous type, of β chemokines that cause histamine release and in increased production of α chemokines that inhibit histamine release. Conclusion: Abnormal chemokine production may contribute to the pathogenesis of bronchial asthma, and restoration of normal chemokine production may be used to explain, in part, the clinical efficacy of immunotherapy.
Subjects
asthma; children; GROα; HRF; IL-8; immunotherapy; MCAF; MIP-1α; mite; RANTES
Other Subjects
allergen; beta 2 adrenergic receptor stimulating agent; chemokine; house dust allergen; immunoglobulin E; interleukin 8; macrophage inflammatory protein 1; monocyte chemotactic protein; RANTES; steroid; theophylline; article; asthma; cell culture; child; clinical article; clinical feature; controlled study; Dermatophagoides farinae; disease severity; enzyme linked immunosorbent assay; female; human; human cell; immunotherapy; male; mononuclear cell; priority journal; radioallergosorbent test; skin test
Publisher
Mosby Inc.
Type
journal article