Purification and Identification of Transglutaminase substrates in mouse testis
Date Issued
2007
Date
2007
Author(s)
Chang, Ning-Chuan
DOI
zh-TW
Abstract
TGase (Transglutaminase) was first discovered by Heinrich Waelsch in 1957. It is a calcium-dependent enzyme, which catalyzes the formation of isopeptide linkages between the γ-carboxamide group of a glutamine residue and the ε-amino group of a lysine residue. Some physiological roles are demonstrated for TGase, including blood coagulation, regulation of cell growth and differentiation, and programmed cell death.
Testis is a component of both the reproductive system and the endocrine system. The respective functions of the testis are: producing sperm (spermatozoa) and producing male sex hormones, of which testosterone is the best known . The process of spermatogenesis takes approximately 70 days, it starts at puberty and usually continues uninterruptedly until death, although a slight decrease in the quantity of produced sperm can be discerned with increase in age.
In this research, we adopted proteomics methods to purify and identify TGase substrates. After MS/MS analysis, we have identified over 70 potential TGase substrates, some of them have been verified in our lab by in vitro transamidation of recombinant TGase substrates and/or by immunoblotting of TGase substrates in a pull-down assay. More importantly, most of the identified TGase substrates are novel and have not yet been studied. Our future work will be to examine the effects of transamidation on the feature of each novel substrate, in terms of enzyme activity, protein-protein interaction, and protein translocation.
Testis is a component of both the reproductive system and the endocrine system. The respective functions of the testis are: producing sperm (spermatozoa) and producing male sex hormones, of which testosterone is the best known . The process of spermatogenesis takes approximately 70 days, it starts at puberty and usually continues uninterruptedly until death, although a slight decrease in the quantity of produced sperm can be discerned with increase in age.
In this research, we adopted proteomics methods to purify and identify TGase substrates. After MS/MS analysis, we have identified over 70 potential TGase substrates, some of them have been verified in our lab by in vitro transamidation of recombinant TGase substrates and/or by immunoblotting of TGase substrates in a pull-down assay. More importantly, most of the identified TGase substrates are novel and have not yet been studied. Our future work will be to examine the effects of transamidation on the feature of each novel substrate, in terms of enzyme activity, protein-protein interaction, and protein translocation.
Subjects
轉麩胺醯胺脢
睪丸
Transglutaminase
Testis
Type
other
File(s)![Thumbnail Image]()
Loading...
Name
ntu-96-R94b46013-1.pdf
Size
23.31 KB
Format
Adobe PDF
Checksum
(MD5):5972c297a518c2e646d9656be3a4419c