Fermented soy paste alleviates lipid accumulation in the liver by regulating the AMPK pathway and modulating gut microbiota in high-fat-diet-fed rats
Journal
Journal of agricultural and food chemistry
Journal Volume
68
Journal Issue
35
Pages
9345-9357
Date Issued
2020
Author(s)
Tung, Y.-C.
Liang, Z.-R.
Chou, S.-F.
Ho, C.-T.
Kuo, Y.-L.
Cheng, K.-C.
Lu, T.-J.
Chang, Y.-C.
Abstract
Nonalcoholic fatty liver disease (NAFLD) is the most common cause of liver disease due to lipid accumulation in the hepatocyte. Diet, especially a high-fat diet, is one risk factor that leads to NAFLD. Many natural compounds such as isoflavones have antiobesity effects. Therefore, intake of these functional compounds through daily dietary choices is a method of improving health. Miso is a kind of fermented soy paste, which is rich in isoflavones and has a different biological activity. In this study, we investigated the effects of different concentrations of fermented soy paste on NAFLD in high-fat-diet (HFD)-fed Sprague-Dawley (SD) rats. The results showed that 2% fermented soy paste decreased serum triacylglycerol (TG) and alanine aminotransferase (ALT) and reduced lipid accumulation in the liver through induced fatty acid oxidation by activating the adenosine 5′-monophosphate -activated protein kinase (AMPK) pathway and increasing PGC1α and CPT1α protein expression. Furthermore, we found that 2% fermented soy paste increased the abundance of Prevotellaceae NK3B31 and Desulfovibrio. Taken together, fermented soy paste improved HFD-induced lipid accumulation in the liver by activating fatty acid oxidation and modulating gut microbiota.
SDGs
Other Subjects
Amino acids; Bioactivity; Chemical activation; Flavonoids; Proteins; Rats; Activated proteins; Alanine aminotransferase; Fatty acid oxidation; Functional compounds; Lipid accumulations; Natural compounds; Non-alcoholic fatty liver disease; Protein expressions; Fatty acids; alanine aminotransferase; fatty acid; hydroxymethylglutaryl coenzyme A reductase kinase; peroxisome proliferator activated receptor gamma coactivator 1alpha; Ppargc1a protein, rat; triacylglycerol; adverse event; animal; enzymology; genetics; human; intestine flora; lipid diet; lipid metabolism; liver; male; metabolism; microbiology; nonalcoholic fatty liver; rat; soy food; Sprague Dawley rat; Alanine Transaminase; AMP-Activated Protein Kinases; Animals; Diet, High-Fat; Fatty Acids; Gastrointestinal Microbiome; Humans; Lipid Metabolism; Liver; Male; Non-alcoholic Fatty Liver Disease; Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; Rats; Rats, Sprague-Dawley; Soy Foods; Triglycerides
Type
journal article