Anti-Carbohydrate Antibodies Elicited by Polyvalent Display on a Viral Scaffold
Resource
ChemBioChem, 8(12), 1455-1462
Journal
ChemBioChem
Pages
1455-1462
Date Issued
2007
Date
2007
Author(s)
Kaltgrad, Eiton
Gupta, Sayam Sen
Punna, Sreenivas
Huang, Cheng-Yuan
Chang, Aileen
Wong, Chi-Huey
Finn, M. G.
Blixt, Ola
Abstract
Tetra- and hexasaccharides were arrayed on the exterior surface of cowpea mosaic virus by using a copper-catalyzed azide-alkyne cycloaddition reaction. Inoculation of chickens with these virus conjugates gave rise to large quantities of polyclonal antiglycan IgY antibodies that displayed excellent avidity and specificity on analysis with printed glycan microarrays. Avian IgY antibodies are produced in significantly higher yield than is possible for mouse or rabbit IgG, and exhibit reduced cross reactivity with native mammalian proteins. For a tri-LacNAc antigen, affinity purification against immobilized mono-LacNAc was necessary to provide a set of antibodies with specific binding properties. Comparable performance was observed for the virus-based polyclonal versus a commercial monoclonal antibody raised against the globo-H tetrasaccharide; this highlights the utility of the glycan microarray for both quality control and rapid in-depth analysis. Virus-carbohydrate conjugates are promising candidates for development in diagnostic and immunotherapeutic applications. © 2007 Wiley-VCH Verlag GmbH & Co. KGaA.
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