Investigation of Genetic Diversity and Traits Related to Seed Dormancy in Rice Collections in Taiwan
Date Issued
2014
Date
2014
Author(s)
Yeh, Jen-Hau
Abstract
RAD (Restriction-site associated DNA) sequencing is a cost-efficient genotyping technique capabable of discovering substaintial genome-wide SNP markers and determining their genotypes simultaneously for selected DNA samples. In current study, 425 rice (Oryza Sativa L.) accessions mainly collected from agricultural experimental stations in Taiwan were genotyped using the RAD sequencing technique. Genotypic data from 14,377 SNP markers of 425 rice accessions were used to investigate genetic diversity and population structure. Seed dormancy and pre-harvest sprouting were measured for the same rice collections at NTU farm in the second season in 2013. In combination of the aforementioned genetic and phenotypic data, QTL mapping were conducted using GWAS (genome-wide association study). The result showed that the genetic compositions of these 425 rice collections were derived from three ancenstral groups: indica rice, temperate japonica rice, and tropical japonica rice. For rice collections possessing genetic background mainly from temperate japonica rice, their genetic components could be further divided into two groups, one for old varieties and the other for modern varieties. In addition, three japonica varieties KHY4625, B6490, and NKY 982117 were characterized for low pre-harvest sprouting rate and low seed dormancy, and are ideal parental lines to breed rice varieties with such characteristics. Futhermore, at least one significant QTL associated with pre-harvest sprouting were detected at the distal end of chromosome 1 in japonica subspicies.
Subjects
RAD定序法
穗上發芽
種子休眠性
族群結構
全基因組關聯性分析
Type
thesis
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